Corticosterone Infused Intracerebroventricularly Inhibits Energy Storage and Stimulates the Hypothalamo-Pituitary Axis in Adrenalectomized Rats Drinking Sucrose

• DOI: 10.1210/en.2002-220613
• Publication Date: 2002-11-23T05:26:31Z
• Authors: Kevin D. Laugero; Francisca Gomez; Sotara Manalo; Mary F. Dallman
• Keywords: Corticosterone; Basal (medicine); Corticotropin-releasing hormone

When allowed to drink sucrose, bilaterally adrenalectomized (ADX) rats exhibit normal weight gain, food intake, sympathetic neural activity, and ACTH compared with sham-ADX rats. Furthermore, ADX drinking sucrose have corticotropin-releasing factor (CRF) mRNA throughout brain. In without all of these variables are abnormal. Systemic corticosterone (B) replacement also restores in normal. To test whether B acts centrally, we infused or saline intracerebroventricularly into under basal conditions after repeated restraint. Rats were exposed no stress 3 h/d restraint for d. Body weights fluid intakes measured. Brains analyzed using immunocytochemistry against glucocorticoid receptors (GR) CRF. Intracerebroventricular blocked the positive effects on metabolism, increased concentrations, augmented responses d 3. B-infused exhibited nuclear GR staining perirhinal cortex, hippocampus, hypothalamic paraventricular nuclei, showing that spreads effectively. CRF nucleus hypothalamus was higher B- than saline-infused We conclude systemically, but not brain, restore metabolism neuropeptides adrenalectomy. By contrast, tonic occupancy brain initiates metabolic characteristic stress.