Cellular and Molecular Characterization of the Adipose Phenotype of the Aromatase-Deficient Mouse

• DOI: 10.1210/en.2002-221123
• Publication Date: 2003-03-15T13:56:18Z
• Authors: Marie L. Misso; Yoko Murata; Wah Chin Boon; Margaret E. E. Jones; Kara L. Britt; Evan R. Simpson
• Keywords: Hormone-sensitive lipase

Estrogen deficiency in the aromatase knockout (ArKO) mouse leads to development of obesity by as early 3 months age, which is characterized a marked increase weights gonadal and infrarenal fat pads. Humans with natural mutations gene also develop metabolic syndrome. In present study cellular molecular parameters were investigated adipose tissue from 10-wk-old wild-type (WT) ArKO female mice treated 17beta-estradiol or placebo identify basis for intraabdominal obesity. Stereological examination revealed that adipocytes isolated significantly larger more abundant than WT mice. Upon treatment estrogen, volume these was greatly reduced, whereas reduction number much less pronounced. Transcriptional analysis using real-time PCR concomitant changes adipocyte levels transcripts encoding leptin lipoprotein lipase, peroxisome proliferator-activated receptor gamma followed pattern closer number. Little change observed factors involved de novo fatty acid synthesis, beta-oxidation, lipolysis, suggesting uptake lipids circulation are main mechanisms estrogen regulates lipid metabolism