Estrogen Stimulates Estrogen-Related Receptor α Gene Expression through Conserved Hormone Response Elements

• DOI: 10.1210/en.2003-0432
• Publication Date: 2003-07-29T00:26:38Z
• Authors: Dianxin Liu; Zhiping Zhang; Wesley Gladwell; Christina T. Teng
• Keywords: Hormone response element; Estrogen receptor beta; Response element; Estrogen receptor alpha; Chromatin immunoprecipitation; Estrogen-related receptor alpha

The estrogen-related receptor alpha gene encodes a nuclear protein, ERR alpha, whose structure is closely related to the estrogen receptors. modulates (ER)-mediated signaling pathways both positively and negatively. It selectively expressed in variety of cell types during development adult tissues. We have previously shown that stimulates expression mouse uterus. In this study, we found stimulated by uterus heart but not liver. Estrogen also human breast endometrial lines. promoter contains multiple Sp1 binding sites, protein required for activity. major response mediated 34-bp DNA element steroid hormone half-sites (MHREs) are conserved between promoters. Mutations made at single or sites MHREs abolished ER-mediated transcription transient transfection experiments. By chromatin immunoprecipitation assay, demonstrated interaction ER endogenous MCF-7 cells. treatment further enhanced association vivo. present study downstream target alpha.