Galanin-Like Peptide Stimulates the Release of Gonadotropin-Releasing Hormonein Vitroand May Mediate the Effects of Leptin on the Hypothalamo-Pituitary-Gonadal Axis

• DOI: 10.1210/en.2003-0873
• Publication Date: 2003-10-28T07:45:00Z
• Authors: Asha Seth; Sarah Stanley; Preeti Jethwa; James Gardiner; Mohammad Ghatei; Stephen Bloom
• Keywords: Galanin receptor; Kisspeptin; Leptin receptor

Leptin regulates the hypothalamo-pituitary-gonadal axis in relation to nutritional status. The mechanism through which leptin mediates its effects on neuroendocrine reproductive circuits remains unclear. Galanin-like peptide (GALP) is a recently identified hypothalamic peptide, localized arcuate nucleus, seems be regulated by and stimulates LH when administered centrally. Here, we demonstrate that release of GALP GnRH vitro from explants harvested male rats. In addition, show GT1-7 cells. Furthermore, antiserum blocks stimulatory action explants. ligand galanin receptors. We therefore investigated whether effect may mediated via known receptor. GALP-stimulated was attenuated (but not abolished) receptor antagonist galantide. However, cells diminished coadministration none cloned receptors were expressed RT-PCR. These observations suggest stimulate an indirect pathway involving direct neurons, possibly novel findings mediate actions provide link between nutrition fertility.