Induction of Adiponectin in Skeletal Muscle by Inflammatory Cytokines:in Vivoandin VitroStudies

• DOI: 10.1210/en.2004-0503
• Publication Date: 2004-08-20T03:02:35Z
• Authors: Aurélie M. Delaigle; Jean-Christophe J...; Isabelle B. Bauche; Olivier Cornu; Sonia M. Brichard
• Keywords: Proinflammatory cytokine

Adiponectin (ApN) is an adipocytokine that plays a fundamental role in energy homeostasis and counteracting inflammation. We examined whether ApN could be induced nonadipose tissue, the skeletal muscle, vivo, cultured myotubes response to lipopolysaccharides or proinflammatory cytokines. next explored underlying mechanisms. In injection of mice caused, after 24 h, approximately 10-fold rise mRNA abundance concomitant 70% increase levels tibialis anterior muscle. This induction was reproduced C2C12 for 48 h with cytokine combination, interferon-gamma + TNFalpha. effect occurred time- dose-dependent manner. Several pieces evidence suggest nitric oxide (NO) mediates this up-regulation by cytokines First, vitro exclusively experimental conditions stimulated NO production. Second, inducible synthase production clearly preceded induction. Third, preventing inhibitors synthases, nitro-L-arginine methyl ester NG-methyl-L-arginine, suppressed inductive vivo. Finally, human myotubes. conclusion, our data provide adiponectin up-regulated vivo rodent inflammatory stimuli. The mechanisms seem involve NO-dependent pathway. overexpression may viewed as local antiinflammatory protection way deliver extra supplies during