Development and Function of the Adult Generation of Leydig Cells in Mice with Sertoli Cell-Selective or Total Ablation of the Androgen Receptor
Male
Mice, Knockout
Microscopy
0303 health sciences
571
Sertoli Cells
572
Knockout
Age Factors
Leydig Cells
Cell Count
Inbred C57BL
Electron
Androgen
Mice, Inbred C57BL
Mice
Microscopy, Electron
03 medical and health sciences
Receptors, Androgen
Receptors
Testis
Androgens
Animals
Female
DOI:
10.1210/en.2005-0300
Publication Date:
2005-05-27T00:25:04Z
AUTHORS (13)
ABSTRACT
Abstract It is established that androgens and unidentified Sertoli cell (SC)-derived factors can influence the development of adult Leydig cells (LC) in rodents, but mechanisms are unclear. We evaluated LC function SC-selective androgen receptor (AR) knockout (SCARKO) complete AR (ARKO) mice. In controls, number increased 26-fold size by approximately 2-fold between 12 140 d age. SCARKOs was normal on 12, reduced more than 40% at later ages, although were larger contained lipid droplets mitochondria control adulthood. ARKO up to 83% all ages compared with did not increase beyond 12. Serum LH testosterone levels seminal vesicle weights comparable whereas elevated 8-fold ARKOs, appeared normal. Immunohistochemistry quantitative PCR for LC-specific markers indicated steroidogenic per probably ARKOs. SCARKOs, insulin-like factor-3 estrogen sulfotransferase (EST) mRNA expression unchanged 3-fold, respectively, both 90% Changes EST expression, coupled platelet-derived growth factor-A potential causes altered SCARKOs. These results show loss action SC has major consequences development, this could be mediated indirectly via and/or estrogens/EST.
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