Molecular Cloning and Spatiotemporal Expression of Prostaglandin F Synthase and Microsomal Prostaglandin E Synthase-1 in Porcine Endometrium

• DOI: 10.1210/en.2005-0880
• Publication Date: 2005-10-14T00:25:23Z
• Authors: Agnieszka Waclawik; Adolfo Rivero-Muller; Agnieszka Blitek; Monika M. Kaczmarek; Leon J. S. Brokken; Kikuko Watanabe; Nafis A. Rahman; Adam J. Ziecik
• Keywords: Cloning (programming)

Endometrial prostaglandins (PGs) and the PGE2/PGF2alpha ratio play an important role in regulating estrous cycle establishment of pregnancy. The enzymes downstream cyclooxygenase-2 may determine porcine uterus. Thus, we have cloned PGF synthase (PGFS) microsomal PGE synthase-1 (mPGES-1) characterized their expression endometrium during early PGFS mPGES-1 amino acid sequences possessed a high degree (>67% >77%, respectively) identity with other mammalian homologs. There was little modulation throughout cycle; however, highly up-regulated around time luteolysis. During pregnancy, at protein level showed time-dependent increase (low on d 10-13, intermediate 14-23, 24-25). In 10-11 low 14-17 then increased after 22, reaching maximum 24-25. Immunohistochemistry localization proteins mainly luminal glandular epithelium. Concluding, spatiotemporal indicates involvement luteolysis pig. comparison endometrial 10-13 pregnancy suggest supportive these determining uterine maternal recognition Moreover, both PG synthases initiation implantation indicate significant placentation.