Proteomic Profiling of Cold Thyroid Nodules

• DOI: 10.1210/en.2006-0752
• Publication Date: 2006-12-29T01:24:32Z
• Authors: Kerstin Krause; Stefan Karger; Angelika Schierhorn; Sylvie Poncin; Marie-Christine Many; Dagmar Fuhrer; Dagmar Führer-Sakel
• Keywords: Thyroglobulin; Thyroid Nodules; Sodium-iodide symporter

Cold thyroid nodules (CTNs) represent a frequent endocrine disorder accounting for up to 85% of in population living an iodine-deficient area. Benign CTNs need be distinguished from cancer, which is relatively rare. The molecular etiology benign unresolved. To obtain novel insights into their pathogenesis, protein expression profiling was performed series 27 solitary (10 follicular adenoma and 20 adenomatous nodules) surrounding normal tissues using two-dimensional gel electrophoresis combined with mass spectrometry analysis, Western blotting, immunohistochemistry. proteome analysis revealed specific fingerprint up-regulation three functional systems: 1) cell proliferation, 2) turnover thyroglobulin, 3) H2O2 detoxification. blot immunohistochemistry confirmed the data showed that exhibit significant proteins involved hormone synthesis yet are deficient T4-containing thyroglobulin. This consequential intranodular iodide deficiency, mainly due cytoplasmic sodium symporter localization, portrays CTN as activated proliferating lesion hypothyroid milieu. Furthermore, we provide preliminary evidence generation could override antioxidative system resulting oxidative stress, suggested by finding raised 8-oxo-guanidine DNA adduct formation CTNs.