Expression of Interferon (IFN)-Stimulated Genes in Extrauterine Tissues during Early Pregnancy in Sheep Is the Consequence of Endocrine IFN-τ Release from the Uterine Vein

• DOI: 10.1210/en.2007-0863
• Publication Date: 2007-12-07T01:39:52Z
• Authors: João F. Oliveira; Luiz E. Henkes; Ryan L. Ashley; Scott H. Purcell; Natalia P. Smirnova; D. N. Rao Veerama...; Russell V. Anthony; Thomas R. Hansen
• Keywords: Conceptus; Interferon-stimulated gene; Luteolysis; ISG15

The ruminant conceptus synthesizes and secretes interferon (IFN)-τ, which presumably acts via an intrauterine paracrine mechanism to signal maternal recognition of pregnancy. aims this study were determine whether IFN-stimulated genes (ISG) such as ISG15 OAS-1 are differentially expressed in blood cells circulating the uterus ewes; extrauterine components reproductive tract corpus luteum (CL) also express mRNA for these ISG, antiviral activity is greater uterine vein than artery during early concentrations both ISG significantly (P < 0.0001) endometrium jugular 15-d pregnant ewes nonpregnant ewes. 0.05) CL from Immunohistochemistry revealed intense staining large luteal on d 15 Blood had similar concentrations, suggesting that not conditioned by IFN-τ within uterus. By using assay, venous was found contain 500- 1000-fold higher bioactive arterial It concluded releases IFN-τ, induces tissues time