New Enzymes Involved in the Mechanism of Action of Epidermal Growth Factor in a Clonal Strain of Leydig Tumor Cells

• DOI: 10.1210/en.2007-1580
• Publication Date: 2008-04-04T01:36:17Z
• Authors: Rocío Castilla; Mariana Gadaleta; Ana Fernanda Cast...; Alejandra Duarte; Isabel Neuman; Cristina Paz; Fabiana Cornejo M...; Ernesto J. Podestá
• Keywords: Male; 0303 health sciences; Arachidonic Acid; Epidermal Growth Factor; Reverse Transcriptase Polymerase Chain Reaction; Blotting, Western; Leydig Cells; Phosphoproteins; Clone Cells; Mitochondria; Rats; 03 medical and health sciences; Cell Line, Tumor; Coenzyme A Ligases; Animals; Thiolester Hydrolases; Cells, Cultured; Leydig Cell Tumor

The studies presented herein were designed to investigate the effect of mouse epidermal growth factor (mEGF) on arachidonic acid (AA) release in a clonal strain cultured murine Leydig cells (designed MA-10). In MA-10 cells, mEGF promotes AA and metabolism lipoxygenated products induce steroidogenic acute regulatory (StAR) protein. However, mechanism by which releases these is not totally elucidated. We show that produces an increment mitochondrial content short-term incubation (30 min). This released action acyl-CoA thioesterase (Acot2), as demonstrated experiments Acot2 was down or overexpressed. turn regulates StAR protein expression, indirect evidence its products. also induces expression (mRNA protein) synthetase provides substrate, arachidonyl-CoA, Acot2. observed another cell line, adrenocortical Y1 cells. Taken together, our results that: 1) can generation specific compartment i.e. mitochondria; 2) up-regulate mRNA levels; 3) mEGF-stimulated intramitochondrial leads induction.