New Enzymes Involved in the Mechanism of Action of Epidermal Growth Factor in a Clonal Strain of Leydig Tumor Cells

Male 0303 health sciences Arachidonic Acid Epidermal Growth Factor Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Leydig Cells Phosphoproteins Clone Cells Mitochondria Rats 03 medical and health sciences Cell Line, Tumor Coenzyme A Ligases Animals Thiolester Hydrolases Cells, Cultured Leydig Cell Tumor
DOI: 10.1210/en.2007-1580 Publication Date: 2008-04-04T01:36:17Z
ABSTRACT
The studies presented herein were designed to investigate the effect of mouse epidermal growth factor (mEGF) on arachidonic acid (AA) release in a clonal strain cultured murine Leydig cells (designed MA-10). In MA-10 cells, mEGF promotes AA and metabolism lipoxygenated products induce steroidogenic acute regulatory (StAR) protein. However, mechanism by which releases these is not totally elucidated. We show that produces an increment mitochondrial content short-term incubation (30 min). This released action acyl-CoA thioesterase (Acot2), as demonstrated experiments Acot2 was down or overexpressed. turn regulates StAR protein expression, indirect evidence its products. also induces expression (mRNA protein) synthetase provides substrate, arachidonyl-CoA, Acot2. observed another cell line, adrenocortical Y1 cells. Taken together, our results that: 1) can generation specific compartment i.e. mitochondria; 2) up-regulate mRNA levels; 3) mEGF-stimulated intramitochondrial leads induction.
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