Adaptative Nitric Oxide Overproduction in Perivascular Adipose Tissue during Early Diet-Induced Obesity

• DOI: 10.1210/en.2009-1464
• Publication Date: 2010-04-21T21:39:59Z
• Authors: Marta Gil-Ortega; Paula Stucchi; Rocío Guzmán-Ruiz; Victoria Cano; Silvia Arribas; M. Carmen González; Mariano Ruiz-Gayo; Maria S. Fernánd...; Beatriz Somoza
• Keywords: Mesenteric arteries; Sodium nitroprusside; Electrical impedance myography; Endothelial Dysfunction

Perivascular adipose tissue (PVAT) plays a paracrine role in regulating vascular tone. We hypothesize that PVAT undergoes adaptative mechanisms during initial steps of diet-induced obesity (DIO) which contribute to preserve function. Four-week-old male C57BL/6J mice were assigned either control [low-fat (LF); 10% kcal from fat] or high-fat diet (HF; 45% fat). After 8 wk dietary treatment function was analyzed the whole perfused mesenteric bed (MB) and isolated arteries cleaned PVAT. Relaxant responses acetylcholine (10−9–10−4m) sodium nitroprusside (10−12–10−5m) significantly ameliorated MB HF animals. However, there no difference between LF groups devoid The enhancement relaxant detected not attributable an increased release nitric oxide (NO) endothelium nor sensitivity and/or activity muscular guanilylcyclase. Mesenteric animals showed bioavailability NO, by 4,5-diaminofluorescein diacetate (DAF2-DA) staining, positively correlated with plasma leptin levels. DAF-2DA staining absent ob/ob but these after 4-wk replacement. main finding this study is NO overproduction occurs early DIO might be aimed at preserving