Circadian Feeding Drive of Metabolic Activity in Adipose Tissue and not Hyperphagia Triggers Overweight in Mice: Is There a Role of the Pentose-Phosphate Pathway?

• DOI: 10.1210/en.2011-1023
• Publication Date: 2011-12-07T04:40:22Z
• Authors: Paula Stucchi; Marta Gil-Ortega; Beatriz Merino; Rocío Guzmán-Ruiz; Victoria Cano; Ismael Valladolid...; Beatriz Somoza; Sophie Le Gonidec; Jesús Argente; Philippe Valet; Julie Ann Chowen; Marisol Fernández...; Mariano Ruiz-Gayo
• Keywords: Lipogenesis; Fatty acid synthesis

High-fat (HF) diets trigger an increase in adipose tissue and body weight (BW) disordered eating behavior. Our study deals with the hypothesis that circadian distribution of energy intake is more relevant for BW dynamics than diet composition. Four-week-old mice were exposed 8 wk to a HF compared animals receiving control chow. progressively increased BW, decreased amount nocturnal (1800-0900 h) calories (energy or food intake) (30%) diurnal (0900-1800 caloric intake), although total daily was identical between groups. Animals killed at 3-h intervals plasma insulin, leptin, corticosterone, glucose, fatty acid levels quantified. Adipose weighed, enzymatic activities integral pentose phosphate pathway (PPP) assayed lumbar tissue. Phosphorylated AMP-dependent protein kinase synthase quantified by Western blotting. In mice, there shift oscillations parameters together inhibition PPP activity decrease phosphorylated synthase. second experiment, forced adhere pattern similar animals. this case, tissue, morning appeared be normal. These data indicate feeding behavior can gain independently composition intake. Because main source reduced nicotinamide adenine dinucleotide phosphate, we suggest might early marker dysfunction diet-induced obesity.