Knockdown of Type 3 Iodothyronine Deiodinase Severely Perturbs Both Embryonic and Early Larval Development in Zebrafish

• DOI: 10.1210/en.2013-1660
• Publication Date: 2014-01-27T17:49:11Z
• Authors: Marjolein Heijlen; Anne M. Houbrechts; Enise Bagci; Stijn L.J. Van Herck; Simone Kersseboom; Camila V. Esguerra; Ronny Blust; Theo J. Visser; Dries Knapen; Veerle M. Darras
• Keywords: Morpholino; Iodothyronine deiodinase; DIO2; Otic vesicle

Exposure to appropriate levels of thyroid hormones (THs) at the right time is key importance for normal development in all vertebrates. Type 3 iodothyronine deiodinase (D3) prime TH-inactivating enzyme, and its expression highest early stages vertebrate development, implying that it may be necessary shield developing tissues from overexposure THs. We used antisense morpholino knockdown examine role D3 during zebrafish. Zebrafish possess 2 genes, dio3a dio3b. Here, we show both genes are expressed contribute vivo activity. However, dio3b mRNA embryos higher, effects on activity resulting phenotype more severe. induced an overall delay as determined by measurements otic vesicle length, eye ear size, body length. The hatching was also severely delayed D3-knockdown embryos. Importantly, observed a severe disturbance several aspects development. Swim bladder inflation aberrant liver intestine. Furthermore, larvae spent significantly less moving, exhibited perturbed escape responses, suggesting affects muscle and/or functioning. These data indicate essential zebrafish embryonic larval value this model further elucidate specific some