Assessment of the Metabolic Pathways Associated With Glucose-Stimulated Biphasic Insulin Secretion

• DOI: 10.1210/en.2013-1805
• Publication Date: 2014-02-24T20:43:52Z
• Authors: Mei Huang; Jamie W. Joseph
• Keywords: Sarcosine

Biphasic glucose-stimulated insulin secretion involves a rapid first phase followed by prolonged second of secretion. The biochemical pathways that control these 2 phases are poorly defined. In this study, we used gas chromatography mass spectroscopy-based metabolomics approach to perform global analysis cellular metabolism during biphasic A time course metabolomic the clonal β-cell line 832/13 cells showed glycolytic, tricarboxylic acid, pentose phosphate pathway, and several amino acids were strongly correlated Interestingly, first-phase was negatively associated with L-valine, trans-4-hydroxy-L-proline, trans-3-hydroxy-L-proline, DL-3-aminoisobutyric L-glutamine, sarcosine, L-lysine, thymine positively L-glutamic flavin adenine dinucleotide, caprylic uridine 5'-monophosphate, phosphoglycerate, myristic capric oleic linoleic palmitoleic acid. Tricarboxylic acid cycle intermediates pyruvate, α-ketoglutarate, succinate second-phase Other metabolites such as myo-inositol, cholesterol, DL-3-aminobutyric L-norleucine These studies provide detailed key either or insights provided data set create framework for planning future in assessment metabolic regulation