Specialized Subpopulations of Kisspeptin Neurons Communicate With GnRH Neurons in Female Mice

• DOI: 10.1210/en.2014-1671
• Publication Date: 2014-10-22T17:22:49Z
• Authors: Devesh Kumar; Michael Candlish; Vinod Periasamy; Nergiz Avcu; Christian Mayer; Ulrich Boehm
• Keywords: Kisspeptin

The neuropeptide kisspeptin is a potent stimulator of GnRH neurons and has been implicated as major regulator the hypothalamus-pituitary-gonadal axis. There are mainly two anatomically segregated populations that express in female hypothalamus: one anteroventral periventricular nucleus (AVPV) other arcuate (ARC). Distinct roles have proposed for AVPV ARC during reproductive maturation mediating estrogen feedback on axis adults. Despite their pivotal role regulation physiology, little known about neuron connectivity. Although previous data suggest heterogeneity within populations, how many which these potential subpopulations actually communicating with not known. Here we used combinatorial genetic transsynaptic tracing strategy to start analyze connectivity individual population mice single-cell resolution. We find only subsets synaptically connected neurons. demonstrate majority does communicate Furthermore, show all sensitive most tyrosine hydroxylase. Our functional specialization populations.