Expression of mRNA for somatostatin receptor (sstr) types 2 and 5 in individual rat pituitary cells. A double labeling in situ hybridization analysis.

• DOI: 10.1210/endo.136.11.7588263
• Publication Date: 2014-01-08T11:06:45Z
• Authors: R Day; W Dong; R Panetta; J Kraicer; M T Greenwood; Y C Patel
• Keywords: Corticotropic cell; Thyrotropic cell; Prolactin cell

To characterize cell specific expression of sstr subtypes in the pituitary we have analyzed mRNA for sstr1-5 rat somatotrophs by reverse transcriptase polymerase chain reaction and determined pattern level 2 5 individual subpopulations double label situ hybridization. Purified expressed all sstrs. In hybridization analysis revealed sstr5 70% somatotrophs, 57% thyrotrophs, 38% corticotrophs, 33% lactotrophs, 21% gonadotrophs. sstr2 occurred 40% 36% 26% 3% 8% Not only were more cells positive but average number autoradiographic grains/cell was also higher than sstr2. These results show multiple genes cells. occur each major subsets, being widely abundantly