Histidine Decarboxylase Gene in the Mouse Uterus Is Regulated by Progesterone and Correlates with Uterine Differentiation for Blastocyst Implantation*

• DOI: 10.1210/endo.139.9.6173
• Publication Date: 2014-01-08T16:09:28Z
• Authors: B. C. Paria; N. Das; S. K. Das; X. Zhao; K. N. Dileepan; S. K. Dey
• Keywords: Histidine decarboxylase; Northern blot; Progesterone receptor

Cell-cell interactions between the blastocyst trophectoderm and uterine luminal epithelium are essential to process of implantation. The factors that participate in these or their mechanism actions poorly understood. Histamine has long been suspected as one is involved formed from L-histidine by histidine decarboxylase (HDC). We examined expression regulation HDC gene mouse uterus during early pregnancy under steroid hormonal stimulation. Northern blot hybridization detected a 2.6-kb transcript messenger RNA (mRNA) poly(A)+ samples. Maximum accumulation mRNA occurred on days 3 4 pregnancy, followed marked declines later (days 5-8). In ovariectomized mice, levels were up-regulated an injection progesterone (P4) 6 h, maintained through 24 h. contrast, estradiol-17beta neither stimulated nor antagonized P4-induced accumulation. up-regulation was considerably abrogated pretreatment with RU-486, P4 receptor antagonist, suggesting involvement receptor. situ specifically epithelial cells but not other cell types. Again, high day pregnancy. With progression implantation 5-8), declined surrounding implanting blastocysts, compared away blastocysts. Immunoreactive histamine colocalized mRNA. Western blotting 54-kDa protein extracts, which also exhibited activity. Expression cells, preceding 4, at lower after initiation 5, its suggest this plays important role