Extracellular Matrix-Associated Bone Morphogenetic Proteins Are Essential for Differentiation of Murine Osteoblastic Cellsin Vitro1

• DOI: 10.1210/endo.140.5.6704
• Publication Date: 2014-01-08T16:09:48Z
• Authors: Miyuki Suzawa; Yasuhiro Takeuchi; Seiji Fukumoto; Shigeaki Kato; Naoto Ueno; Kohei Miyazono; Toshio Matsumoto; Toshiro Fujita
• Keywords: BMPR2

Osteoblastic differentiation is an essential part of bone formation that compensates resorbed matrix to maintain its structural integrity. Cells in osteoblast lineage develop differentiated phenotypes during a long-term culture vitro. However, intrinsic mechanisms whereby these cells differentiate into mature osteoblasts are yet unclear. Bone morphogenetic proteins (BMPs) stimulate osteoblastic and formation. We demonstrate mouse MC3T3-E1 constitutively expressed messenger RNAs (mRNAs) for BMP-2 BMP-4 accumulated BMPs collagen-rich extracellular matrices. associated with the matrices were involved induction nonosteogenic mesenchymal as well lineage. type IA II BMP receptors. When kinase-deficient receptor was stably transfected obliterate BMP-2/4 signaling, not only failed respond exogenous but lost their capability form mineralized nodules. These observations strongly suggest endogenous Therefore, regulatory mechanism actions principal issue be elucidated better understanding pathogenesis losing diseases such osteoporosis.