Effects of Intermittent Parathyroid Hormone Administration on Bone Mineralization Density in Iliac Crest Biopsies from Patients with Osteoporosis: A Paired Study before and after Treatment

• DOI: 10.1210/jc.2002-021988
• Publication Date: 2003-03-11T02:02:44Z
• Authors: Barbara M. Misof; Paul Roschger; Felicia Cosman; Etah S. Kurland; Walter Tesch; Phaedra Messmer; David W. Dempster; Jeri Nieves; Elizabeth Shane; Peter Fratzl; Klaus Klaushofer; John Bilezikian; Robert Lindsay
• Keywords: Male; 0301 basic medicine; Biopsy; Middle Aged; Ilium; 03 medical and health sciences; Absorptiometry, Photon; Bone Density; Parathyroid Hormone; Humans; Osteoporosis; Scattering, Radiation; Calcium; Female

Anabolic effects of PTH have been observed at several skeletal sites in humans by dual x-ray absorptiometry without differentiating between an actual increase in bone volume and an increase in mineral content within already established bone. The present study addressed this issue by evaluating the bone mineralization density distribution of iliac crest bone biopsies before and after PTH treatment for 18-36 months in men and women with osteoporosis using quantitative backscattered electron imaging. In cortical bone, pairwise comparison of the two biopsies before and after treatment revealed a reduction in the typical calcium concentration in men (-3.32%; P = 0.02, by paired t test), but no change in women, and the heterogeneity of mineralization increased in both males and females [+18.80% (P = 0.09) and +18.14% (P = 0.005), respectively]. In cancellous bone, there was no change in the typical calcium concentration, but there was a greater heterogeneity of mineralization in both men and women [+19.65% (P = 0.02) and +21.59% (P = 0.056), respectively] due to newly formed bone matrix. Small angle x-ray scattering performed on a subgroup of subjects revealed normal collagen/mineral structure. The findings confirm the observations that PTH stimulates skeletal remodeling, resulting in an increased percentage of newly formed bone matrix of lower mineral density.