In VivoEpinephrine-Mediated Regulation of Gene Expression in Human Skeletal Muscle

• DOI: 10.1210/jc.2003-031733
• Publication Date: 2004-05-05T02:58:02Z
• Authors: Nathalie Viguerie; Karine Clement; Pierre Barbe; Melanie Courtine; Arriel Benis; Dominique Larrouy; Blaise Hanczar; Veronique Pelloux; Christine Poitou; Yadh Khalfallah; Gregory S. Barsh; Claire Thalamas; Jean-Daniel Zucker; Dominique Langin
• Keywords: Adult; Male; 0301 basic medicine; Epinephrine; Proteins; Adrenergic Agonists; Up-Regulation; 3. Good health; Cardiovascular Physiological Phenomena; 03 medical and health sciences; Metabolism; Gene Expression Regulation; Stress, Physiological; Cyclic AMP; Humans; RNA, Messenger; Cyclic AMP Response Element-Binding Protein; Energy Metabolism; Muscle, Skeletal; Oligonucleotide Array Sequence Analysis; Signal Transduction

The stress hormone epinephrine produces major physiological effects on skeletal muscle. Here we determined muscle mRNA expression profiles before and during a 6-h infusion performed in nine young men. Stringent statistical analysis of data obtained using 43,000 cDNA element microarrays showed that 1206 474 genes were up- down-regulated, respectively. Microarray validated reverse transcription quantitative PCR. Gene classification was through mining Ontology annotations, cluster regulated among 14 human tissues, correlation clinical parameter variations. Evidence an autoregulatory control provided by the regulation key cAMP-dependent pathway. Genes with known functional cAMP response elements hormone. impact metabolism illustrated coordinated regulations involved carbohydrate protein metabolisms. Epinephrine had profound effect immunity inflammatory response, previously unappreciated aspect catecholamine action. Information 526 mRNAs corresponded to unknown function. These define molecular signatures action They may contribute understanding alterations observed pathological conditions characterized sympathetic nervous system overdrive.