In VivoEpinephrine-Mediated Regulation of Gene Expression in Human Skeletal Muscle

Adult Male 0301 basic medicine Epinephrine Proteins Adrenergic Agonists Up-Regulation 3. Good health Cardiovascular Physiological Phenomena 03 medical and health sciences Metabolism Gene Expression Regulation Stress, Physiological Cyclic AMP Humans RNA, Messenger Cyclic AMP Response Element-Binding Protein Energy Metabolism Muscle, Skeletal Oligonucleotide Array Sequence Analysis Signal Transduction
DOI: 10.1210/jc.2003-031733 Publication Date: 2004-05-05T02:58:02Z
ABSTRACT
The stress hormone epinephrine produces major physiological effects on skeletal muscle. Here we determined muscle mRNA expression profiles before and during a 6-h infusion performed in nine young men. Stringent statistical analysis of data obtained using 43,000 cDNA element microarrays showed that 1206 474 genes were up- down-regulated, respectively. Microarray validated reverse transcription quantitative PCR. Gene classification was through mining Ontology annotations, cluster regulated among 14 human tissues, correlation clinical parameter variations. Evidence an autoregulatory control provided by the regulation key cAMP-dependent pathway. Genes with known functional cAMP response elements hormone. impact metabolism illustrated coordinated regulations involved carbohydrate protein metabolisms. Epinephrine had profound effect immunity inflammatory response, previously unappreciated aspect catecholamine action. Information 526 mRNAs corresponded to unknown function. These define molecular signatures action They may contribute understanding alterations observed pathological conditions characterized sympathetic nervous system overdrive.
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