Distinct Regulation of Cyclooxygenase-2 by Interleukin-1β in Normal and Endometriotic Stromal Cells

• DOI: 10.1210/jc.2004-1612
• Publication Date: 2005-01-10T23:18:48Z
• Authors: Meng-Hsing Wu; Chu-An Wang; Chen-Chung Lin; Lei-Chin Chen; Wen-Chang Chang; Shaw-Jenq Tsai
• Keywords: Proinflammatory cytokine; Ectopic expression

Aberrant production of cyclooxygenase-2 (COX-2) plays pivotal roles in many pathological processes including tumorigenesis and endometriosis, although the underlying mechanism remains obscure. Herein we report evidence to demonstrate that COX-2 is distinctly regulated by IL-1β normal endometriotic stroma. Ectopic stromal cell at least 100 times more sensitive treatment, compared with its eutopic counterpart. Induction expression endometrial stroma primary due enhancement mRNA stability. In contrast, not only increases stability but also up-regulates promoter activity ectopic mediated via MAPK-dependent phosphorylation cAMP-responding element binding protein. Promoter EMSAs a cAMP response site located −571/−564 critical for IL-1β-induced gene expression. Our results indicate elevation tissues may result from increased sensitivity proinflammatory cytokines such as IL-1β, which consistently present peritoneal fluid endometriosis patients. Distinct regulation play role pathophysiological cancer formation endometriosis.