Adipose Triglyceride Lipase and Hormone-Sensitive Lipase Protein Expression Is Decreased in the Obese Insulin-Resistant State

• DOI: 10.1210/jc.2006-1318
• Publication Date: 2007-03-14T00:38:32Z
• Authors: Johan W. E. Jocken; Dominique Langin; Egbert Smit; Wim H. M. Saris; Carine Valle; Gabby B. Hul; Cecilia Holm; Peter Arner; Ellen E. Blaak
• Keywords: Adipose triglyceride lipase; Hormone-sensitive lipase; Hyperinsulinemia

Obesity is associated with increased triacylglycerol (TAG) storage in adipose tissue and insulin resistance. The mobilization of stored TAG mediated by hormone-sensitive lipase (HSL) the recently discovered triglyceride (ATGL). aim present study was to examine whether ATGL HSL mRNA protein expression are altered insulin-resistant conditions. In addition, we investigated a possible impaired could be reversed period weight reduction.Adipose biopsies were taken from obese subjects (n = 44) wide range resistance, before just after 10-wk hypocaloric diet. determined Western blot quantitative RT-PCR, respectively.Fasting levels degree resistance (using homeostasis model assessment index for resistance) negatively correlated expression, independent age, gender, fat cell size, body composition. Both reduced compared insulin-sensitive (P < 0.05). Weight reduction significantly decreased expression. A positive correlation between decrease leptin level observed. Finally, seem highly correlated, indicating tight coregulation transcriptional control.In subjects, hyperinsulinemia strongly mass. Data on indicated that also other factors (e.g. leptin) relate