Association of Low Sodium-Iodide Symporter Messenger Ribonucleic Acid Expression in Malignant Thyroid Nodules with Increased Intracellular Protein Staining

• DOI: 10.1210/jc.2007-0353
• Publication Date: 2008-07-16T04:18:27Z
• Authors: Ana Karina M. B. ...; Ileana G. S. Rubio; Ana Luiza R. Galrão; Meyer Knobel; Eduardo K. Tomimori; Venâncio A. F. A...; Cristina T. Kanamura; Carlos A. Buchpiguel; Tomoco Watanabe; Celso U. M. Frigu...; Marco A. V. Kulcsar; Geraldo Medeiros-...; Rosalinda Y. A. C...
• Keywords: Immunostaining; Thyroid Nodules; Sodium-iodide symporter

The expression of sodium iodide symporter (NIS) is required for uptake in thyroid cells. Benign and malignant tumors have low uptake. However, previous studies by RT-PCR or immunohistochemistry shown divergent results NIS these nodules.The objective the study was to investigate mRNA transcript levels, compare with TSH receptor proteins expression, localize protein nodules samples their surrounding nonnodular tissues (controls).NIS quantified real-time RT-PCR, proteins, evaluated immunohistochemistry, were examined surgical specimens 12 benign 13 control samples.When compared controls, 83.3% 100% had significantly lower gene expression. Conversely, 66.7% stronger intracellular immunostaining than controls. Low associated strong most frequently detected (100%) (50%; P = 0.005). located at basolateral membrane 24% samples, 8.3% benign, 15.4% nodules. percentage positivity (41.6%) higher (7.7%).We confirmed that reduced staining may be related inability migrate cellular membrane. These explain