TGF-β1 Induces COX-2 Expression and PGE2 Production in Human Granulosa Cells Through Smad Signaling Pathways

• DOI: 10.1210/jc.2013-4100
• Publication Date: 2014-04-08T16:09:13Z
• Authors: Lanlan Fang; Hsun-Ming Chang; Jung-Chien Cheng; Peter C. K. Leung; Ying-Pu Sun
• Keywords: EGF Family of Proteins; 0303 health sciences; Granulosa Cells; Epidermal Growth Factor; Receptor, Transforming Growth Factor-beta Type I; Smad Proteins; Protein Serine-Threonine Kinases; Amphiregulin; Dinoprostone; Epiregulin; Gene Expression Regulation, Enzymologic; Transforming Growth Factor beta1; 03 medical and health sciences; Cyclooxygenase 2; Humans; Intercellular Signaling Peptides and Proteins; Female; Betacellulin; Receptors, Transforming Growth Factor beta; Cells, Cultured; Glycoproteins; Signal Transduction

Cyclooxygenase-2 (COX-2) expression and prostaglandin E2 (PGE2) production have been shown to play key roles in the regulation of ovulation. The TGF-β superfamily members are important molecules that regulate many ovarian functions under normal physiological pathological conditions. TGF-β1 its receptors expressed human granulosa cells. However, date, whether can COX-2 PGE2 production, which turn contribute process ovulation, remains unknown.The objective study was investigate effects on cells.SVOG cells were obtained from women undergoing vitro fertilization immortalized with Simian virus 40 large T antigen. SVOG used effect production.The conducted at an academic research center.mRNA protein levels examined by RT-quantitative real-time PCR Western blotting, respectively. concentrations culture medium measured ELISA.TGF-β1 treatment induced production. inductive abolished inhibition type I receptor (TβRI). In addition, activated phosphorylated mothers against decapentaplegic (Smad)-2 Smad3 signaling pathways. Inhibition Smad pathways small interfering RNA-mediated approaches attenuated TGF-β1-induced production.TGF-β1 inducing through a Smad-dependent pathway