Effects of Recombinant Activins on Steroidogenesis in Human Granulosa-Lutein Cells

• DOI: 10.1210/jc.2014-1223
• Publication Date: 2014-07-25T13:04:13Z
• Authors: Hsun-Ming Chang; Jung-Chien Cheng; Christian Klausen; Elizabeth L. Taylor; Peter C. K. Leung
• Keywords: Activin receptor; Progesterone receptor; Granulosa cell

Exerting a broad range of biological effects in various tissues, activins are homo- or heterodimers activin/inhibin β-subunits (βA, βB, βC, and βE humans). Although A (βAβA), B (βBβB), AB (βAβB), AC (βAβC) have been demonstrated the female reproductive system, little is known about their individual functions ovary.To investigate roles activities regulating steroidogenesis human granulosa cells.Human granulosa-lutein cells obtained from 32 patients undergoing vitro fertilization were used to activin A, B, AB, on expression steroidogenic enzymes steroid production.An academic research center.mRNA protein levels examined by reverse transcription quantitative real-time PCR Western blot analysis, respectively. The production estradiol progesterone was measured enzyme immunoassay.P450 aromatase, FSH receptor, increased, whereas acute regulatory (StAR), LH decreased after treatment with but not AC. induced aromatase/estradiol StAR/progesterone; however, pretreatment enhanced gonadotropins aromatase/estradiol, suppressed StAR/progesterone. Treatment phosphorylation SMA- MAD-related proteins (SMAD2 3), had no such effects. Furthermore, co-culture (1-100 ng/mL) (25 did alter P450 aromatase StAR mRNA levels.Activin similar cells. In contrast, biologically active does act as competitive antagonist.