Epigenetic Reprogramming of Immune Cells in Women With PCOS Impact Genes Controlling Reproductive Function

• DOI: 10.1210/jc.2019-01015
• Publication Date: 2019-08-07T09:04:51Z
• Authors: Danielle Hiam; David Simar; Rhianna Laker; Ali Altıntaş; Melanie Gibson-Helm; Elly Fletcher; Alba Moreno-Asso; Adam J Trewin; Romain Barres; Nigel K Stepto
• Keywords: Reprogramming; Differentially methylated regions; CpG site

Abstract Context Polycystic ovary syndrome (PCOS) is a chronic disease affecting reproductive function and whole-body metabolism. Although the etiology unclear, emerging evidence indicates that epigenetics may be contributing factor. Objective To determine role of global genome-wide epigenetic modifications in specific immune cells PCOS compared with controls whether these could related to clinical features PCOS. Design Cross-sectional study. Participants Women (n = 17) or without 17). Setting Recruited from general community. Main Outcome Measures Isolated peripheral blood mononuclear were analyzed using multicolor flow cytometry methods DNA methylation levels cell-specific fashion. Transcriptomic analyses performed on T helper RNA sequencing reduced representation bisulfite sequencing. Results had lower monocytes (P 0.006) 0.004), cytotoxic B 0.03). Specific analysis women identified 5581 differentially methylated CpG sites. Functional gene ontology enrichment showed genes located at proximity sites belong pathways cell function. However, not altered transcriptomic level. Conclusions It was shown associated gene-specific remodeling type–specific manner. Further investigation warranted reprogramming important determining different phenotypes