Expression and Biological Effects of Endothelin-1 in Human Gonadotropin-Releasing Hormone-Secreting Neurons1

• DOI: 10.1210/jcem.85.4.6565
• Publication Date: 2014-01-08T17:05:32Z
• Authors: Mario Maggi; Tullio Barni; Guido Fantoni; Rosa Mancina; Cinzia Pupilli; Michaela Luconi; Clara Crescioli; Mario Serio; Gabriella B. Vann...
• Keywords: Neurons; Endothelin-3; Endothelin-1; Receptors, Endothelin; Embryo, Mammalian; Receptor, Endothelin A; Receptor, Endothelin B; NEURAL CREST CELLS; CONVERTING ENZYME-1; PRIMARY CULTURES; RECEPTORS; MODULATE; PROTEIN; GENE; Gonadotropin-Releasing Hormone; 03 medical and health sciences; 0302 clinical medicine; Olfactory Mucosa; Culture Media, Conditioned; Humans; Calcium; Cells, Cultured; In Situ Hybridization

In a previous report, we demonstrated that in FNC-B4 cells, derived and characterized from human fetal olfactory epithelium, both sex steroids odorants regulate GnRH secretion. We now report the presence biological activity of endothelin (ET)-1 this GnRH-secreting neuronal cell. By situ hybridization immunohistochemistry, found gene protein expression ET-1 its converting enzyme ECE-1 mucosa cells. The authentic conditioned media cells was further supported by combined RIAs high-performance liquid chromatography studies. Experiments with radiolabeled ET-3 strongly indicated two classes binding sites, corresponding to ETA (16,500 sites/cell) ETB receptors (8,700 sites/cell). Functional studies, using selective analogs, these subserve distinct functions receptor subtype mediated an increase intracellular calcium Conversely, stimulation induced DNA synthesis mitogen-activated kinase p44ERK1 expression. This is first demonstration, vitro model, neuroendocrine role for as regulator neuron activity.