Coxsackievirus-Induced Proteomic Alterations in Primary Human Islets Provide Insights for the Etiology of Diabetes

• DOI: 10.1210/js.2017-00278
• Publication Date: 2017-09-11T18:04:16Z
• Authors: Julius O Nyalwidhe; Glen R Gallagher; Lindsey M Glenn; Margaret A Morris; Pranitha Vangala; Agata Jurczyk; Rita Bortell; David M Harlan; Jennifer P Wang; Jerry L Nadler
• Keywords: Coxsackievirus; Pancreatic Islets

Enteroviral infections have been associated with the development of type 1 diabetes (T1D), a chronic inflammatory disease characterized by autoimmune destruction insulin-producing pancreatic beta cells. Cultured human islets, including cells, can be infected coxsackievirus B4 (CVB4) and thus are useful for understanding cellular responses to infection. We performed quantitative mass spectrometry analysis on cultured primary islets CVB4 identify molecules pathways altered upon Corresponding uninfected controls were included in study comparative protein expression analyses. Proteins significantly differentially regulated challenged virus compared their counterparts. Complementary analyses gene transcripts CVB4-infected over time course validated induction RNA many proteins that increased proteomics studies. Notably, infection results considerable decrease insulin. Genes/proteins modulated during also include those involved activation immune responses, I interferon linked T1D pathogenesis antiviral, cell repair, properties. Our applies identifies target could interventions.