Olfactory Receptor OR51E1 Mediates GLP-1 Secretion in Human and Rodent Enteroendocrine L Cells

• DOI: 10.1210/js.2018-00165
• Publication Date: 2018-10-11T14:03:06Z
• Authors: Ye Eon Han; Chan Woo Kang; Joo Heon Oh; Se Hee Park; Cheol Ryong Ku; Yoon Hee Cho; Mi Kyung Lee; Eun Jig Lee
• Keywords: Peptide YY; Enteroendocrine cell

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY), produced by intestinal enteroendocrine L cells, are important gut hormones that coordinate gastrointestinal physiology, metabolism, appetite. We aimed to investigate the role of olfactory receptor (OR) OR51E1 in GLP-1 PYY secretion. analyzed expression marker protein (OMP), an indicator OR-mediated events nonolfactory systems, human cells. Furthermore, we OMP cell line NCI-H716. To whether odorant-activated OR signaling stimulates secretion, used nonanoic acid, a known ligand. Treatment with 100 μM acid increased secretion 2.32 ± 0.41-fold 1.44 0.10-fold; however, this effect was attenuated on small interfering RNA-mediated knockdown. Oral administration rats resulted 2.89 0.53-fold increase levels reductions blood glucose compared control group. Nonanoic via thereby indicating potential secretion; could be translated into therapeutic approach treating diabetes.