Medullary Thyroid Carcinoma Cell Lines Contain a Self-Renewing CD133+Population that Is Dependent onRETActivity

• DOI: 10.1210/mend.23.12.9997
• Publication Date: 2014-01-08T17:35:09Z
• Authors: Wen Zhu; Tao Hai; Lei Ye; Gilbert J. Cote
• Keywords: Stem cell marker; Immunostaining

Medullary thyroid carcinoma (MTC) is a cancer of the parafollicular C cells commonly caused by an inherited or acquired RET protooncogene mutation. Therapeutic resistance and recurrence disease imply presence stem in MTC. In this study, we sought to identify characterize cell-like The characterization cell properties was performed using immunostaining, flow cytometry, sphere formation assay, rederivation Western blotting, quantitative RT-PCR defined markers neural progenitor cells. role activation assessed through RNA interference knockdown. CD133 positivity identified immunostaining patient Flow cytometry confirmed subpopulation CD133+ two MTC lines. could be expanded passaged multiple times, expressed β-tubulin 3 glial fibrillary acidic protein. MZ-CRC-1 line, which harbors M918T mutation, had greater numbers sphere-forming ability than TT less active C634W Sphere more dependent on activity epidermal growth factor fibroblast factor. Our data support existence stem-like MTC, exhibit features self-renewal lineage differentiation that receptor activity. These findings may provide new insights develop promising therapy for