Anti-neurofascin antibody in patients with combined central and peripheral demyelination

• DOI: 10.1212/wnl.0b013e3182a1aa9c
• Publication Date: 2013-07-25T15:02:24Z
• Authors: Nobutoshi Kawamura; Ryo Yamasaki; Tomomi Yonekawa; Takuya Matsushita; Susumu Kusunoki; Shigemi Nagayama; Yasuo Fukuda; Hidenori Ogata; Dai Matsuse; Hiroyuki Murai; Jun-ichi Kira
• Keywords: Polyradiculoneuropathy; Immunostaining

We aimed to identify the target antigens for combined central and peripheral demyelination (CCPD).We screened by immunohistochemistry immunoblotting using nerve tissues recognized serum antibodies from selected CCPD chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) cases. then measured level of antibody relevant antigen in 7 patients with CCPD, 16 CIDP, 20 multiple sclerosis, Guillain-Barré syndrome, 21 other neuropathies, 23 healthy controls (HC) ELISA cell-based assays HEK293 cells.At initial screening, sera 2 showed cross-like binding sciatic sections at fixed intervals, nearly perfect colocalization neurofascin immunostaining node paranode. recombinant revealed significantly higher mean optical density values group than disease groups HC. Anti-neurofascin positivity rates were 86% 10% 25% 15% 0% neuropathies The assay detected anti-neurofascin 5 CCPD; all others negative. CSF samples examined both positive. In antibody-positive patients, including those a limited response corticosteroids, IV immunoglobulin or plasma exchange alleviated symptoms.Anti-neurofascin is frequently present CCPD. Recognition this may be important, because who are positive respond well exchange.