[Wine-processed Chuanxiong Rhizoma enhances efficacy of aumolertinib against EGFRmutant non-small cell lung cancer xenografts in nude mouse brain].

• DOI: 10.12122/j.issn.1673-4254.2023.03.06
• Publication Date: 2023-03-20
• Authors: Wenwen Niu; Xiaocui Rong; Qiu Zhao; X Liu; Lin Xu; Sijia Li; X Li
• Keywords: Nude mouse

To investigate the effect of wine-processed Chuanxiong Rhizoma (WCR) for enhancing efficacy aumolertinib against xenografts epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC) in brain nude mice.In a co-culture system hCMEC/D3 and PC9 NSCLC cells, aqueous extract WCR (2 mg/mL) combined with (10 20 μmol/L) on apoptosis cells was investigated using flow cytometry. The effects (0.5, 1, 2 transmembrane transport 8 μmol/L examined ABCB1-MDCK monolayer cells. Western blotting used to detect expressions tight junction proteins related blood- barrier integrity. A mouse model bearing xenograft established observe inhibitory (1 mg/g) mg/kg) tumor growth.Compared (20 alone, significantly increased rate by 21% (P < 0.01). treatment obviously apical-basolateral 0.05) lowered expression levels zonula occludens-1, claudin-5 P-glycoprotein 0.05). In tumor-bearing mice, compared produced stronger growth, improved weight loss, prolonged survival time mice Pathological examination showed that alleviated neural injuries brain. Immunohistochemistry revealed reduced ZO-1 mice.WCR shows EGFR-mutant possibly due facilitating downregulating ZO-1, expression.