Proteomic analysis of multiple primary cilia reveals a novel mode of ciliary development in mammals

• DOI: 10.1242/bio.20121081
• Publication Date: 2012-07-06T05:25:53Z
• Authors: Keishi Narita; Hiroko Kozuka-Hata; Yuta Nonami; Hiroko Ao-Kondo; Toshimitsu Suzuki; Hideki Nakamura; Kazuhiro Yamakawa; Masaaki Oyama; Takafumi Inoue; Sen Takeda
• Keywords: Primary (astronomy)

Cilia are structurally and functionally diverse organelles, whose malfunction leads to ciliopathies. While recent studies have uncovered common ciliary transport mechanisms, limited information is available on the proteome of cilia, particularly that sensory subtypes, which could provide insight into their functional developmental diversities. In present study, we performed proteomic analysis unique, multiple 9+0 cilia in choroid plexus epithelial cells (CPECs). The juvenile swine CPEC identified 868 proteins. Among them, 396 were shared with photoreceptor (outer segment), whereas only 152 9+2 flagella. Various signaling molecules enriched a CPEC-specific ciliome subset, implicating multiplicity functions. also included for motility such as Rsph9. CPECs from or adult mouse, Rsph9 was localized subpopulation they non-motile. Live imaging mouse revealed neonatal beat vigorously, waned lost within 1-2 weeks. beating characteristics variable different those typical ependyma, yet an Efhc1-mediated mechanism regulate frequency both types cilia. Notably, ultrastructural presence not but atypical subtypes CPEC. Overall, these results conserved components demonstrated novel mode development mammals.