Angiogenic network formation in the developing vertebrate trunk

• DOI: 10.1242/dev.00733
• Publication Date: 2003-09-09T02:20:58Z
• Authors: Sumio Isogai; Nathan D. Lawson; Saioa Torrealday; Masaharu Horiguchi; Brant M. Weinstein
• Keywords: Dorsal aorta; Vascular network

We have used time-lapse multiphoton microscopy of living Tg(fli1:EGFP)y1 zebrafish embryos to examine how a patterned, functional network angiogenic blood vessels is generated in the early vertebrate trunk. Angiogenic vascular sprouts emerge from longitudinal trunk axial (the dorsal aorta and posterior cardinal vein) two spatially temporally distinct steps. Dorsal aorta-derived form an initial primary segments, followed by emergence vein-derived secondary that interact interconnect dynamically with initiate flow. Using transgenic silent heart mutant embryos, we show gross anatomical patterning this does not require circulation. However, our results suggest circulatory flow dynamics play important role helping determine pattern interconnections between sprouts, thus final arterial or venous identity network. discuss model explain combining genetic programming overall architecture hemodynamic determination patterns.