Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis

• DOI: 10.1242/dev.022616
• Publication Date: 2008-08-25T18:17:59Z
• Authors: Ayano Kawaguchi; Tomoko Ikawa; Takeya Kasukawa; Hiroki R. Ueda; Kazuki Kurimoto; Mitinori Saitou; Fumio Matsuzaki
• Keywords: Progenitor; Fate mapping

Cellular diversity of the brain is largely attributed to spatial and temporal heterogeneity progenitor cells. In mammalian cerebral development, it has been difficult determine how heterogeneous neural cells are, owing dynamic changes in their nuclear position gene expression. To address this issue, we systematically analyzed cDNA profiles a large number single at mid-embryonic stage mouse. By cluster analysis situ hybridization, have identified set genes that distinguishes between apical basal progenitors. Despite relatively homogeneous global expression profiles, progenitors exhibit highly variable patterns Notch signaling components, raising possibility causes division these Furthermore, successfully captured nascent state These are generated shortly after birth from progenitors, show strong major ligand delta-like 1, which soon fades away as migrate ventricular zone. We also demonstrated attenuation signals immediately induces differentiation into Thus, Notch-dependent feedback loop likely be operation maintain both populations.