Differential regulation of imprinting in the murine embryo and placenta by theDlk1-Dio3imprinting control region

• DOI: 10.1242/dev.02726
• Publication Date: 2006-12-14T02:53:36Z
• Authors: Shau-Ping Lin; Phil Coan; Simao Teixeira da...; Herve Seitz; Jerome Cavaille; Pi-Wen Teng; Shuji Takada; Anne C. Ferguson-...
• Keywords: Genomic Imprinting; Imprinting (psychology); Uniparental disomy; MEG3

Genomic imprinting is an epigenetic mechanism controlling parental-origin-specific gene expression. Perturbing the parental origin of distal portion mouse chromosome 12 causes alterations in dosage imprinted genes resulting embryonic lethality and developmental abnormalities both embryo placenta. A 1 Mb domain identified on contains three paternally expressed protein-coding multiple non-coding RNA genes, including snoRNAs microRNAs,expressed from maternally inherited chromosome. An intergenic,parental-origin-specific differentially methylated region, IG-DMR, which unmethylated chromosome, necessary for repression activation RNAs: its absence maternal to behave like one. Here, we characterise consequences this epigenotype switch compare these with phenotypes associated paternal uniparental disomy 12. The results show that defects described embryos can be attributed one cluster alone, not mutant placenta, cause prenatal lethality. In IG-DMR has no phenotypic consequence. Loss occurs but RNAs are repressed This indicates action different placenta suggests control differs two lineages.