Syntabulin, a motor protein linker, controls dorsal determination

• DOI: 10.1242/dev.046425
• Publication Date: 2010-02-12T02:06:20Z
• Authors: Hideaki Nojima; Sophie Rothhämel; Takashi Shimizu; Cheol-Hee Kim; Shigenobu Yonemura; Florence L. Marlow; Masahiko Hibi
• Keywords: Polarity in embryogenesis; Kinesin; Linker

In amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized vegetal pole then transported prospective side of embryo along a microtubule array. The DDs known activate canonical Wnt pathway thereby promote expression genes that induce organizer. Here, by identifying locus maternal-effect ventralized mutant tokkaebi, we show Syntabulin, linker kinesin I motor protein, is essential for determination in zebrafish. We found syntabulin mRNA during oogenesis through Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which necessary establishment animal-vegetal (AV) oocyte polarity. demonstrate Syntabulin translocated from microtubule-dependent manner. Our findings suggest regulates transport DDs, provide evidence link between AV dorsoventral axis formation.