Macrophages define dermal lymphatic vessel calibre during development by regulating lymphatic endothelial cell proliferation

• DOI: 10.1242/dev.050021
• Publication Date: 2010-10-26T11:37:58Z
• Authors: Emma J. Gordon; Sujata Rao; Jeffrey W. Pollard; Stephen L. Nutt; Richard A. Lang; Natasha L. Harvey
• Keywords: Lymphangiogenesis; Lymphatic Endothelium; Lymphatic vessel

Macrophages have been suggested to stimulate neo-lymphangiogenesis in settings of inflammation via two potential mechanisms: (1) acting as a source lymphatic endothelial progenitor cells the ability transdifferentiate into and be incorporated growing vessels; (2) providing crucial pro-lymphangiogenic growth factors proteases. We set out establish whether myeloid lineage are important for development vasculature through either these mechanisms. Here, we provide tracing evidence demonstrate that arise independently during both embryogenesis tumour-stimulated lymphangiogenesis mouse, thus excluding macrophages settings. In addition, dermal PU.1–/– Csf1r–/– macrophage-deficient mouse embryos is hyperplastic owing elevated cell proliferation, suggesting signals act restrain vessel calibre skin development. contrast what has demonstrated inflammation, do not comprise principal factors, including VEGFC VEGFD, embryonic microenvironment, illustrating sources patterning proliferative driving disease-stimulated likely distinct.