Regulation of mouse stomach development and Barx1 expression by specific microRNAs

• DOI: 10.1242/dev.056317
• Publication Date: 2011-02-10T02:00:03Z
• Authors: Byeong-Moo Kim; Janghee Woo; Chryssa Kanellopo...; Ramesh A. Shivdasani
• Keywords: Dicer

Although microRNAs (miRNAs) are postulated to fine-tune many developmental processes, their relationships with specific targets and tissues remain largely undefined. The mesenchymal transcription factor Barx1 controls spleen stomach morphogenesis is required specify stomach-specific epithelium in adjacent endoderm. expression precisely regulated space time, a sharp drop levels after epithelial specification. We tested the hypothesis that miRNAs mediate this marked decline levels. Depletion of miRNA-processing enzyme Dicer cultured mesenchyme conditional gene deletion mice significantly increased levels, disrupted intestine development caused agenesis. Computational experimental studies identified miR-7a miR-203 as candidate regulate expressed inverse proportion it fetal mouse stomach. Through interactions cognate sequences 3′ untranslated region, repress cells its function inducing gastric epithelium. These results indicate for proper digestive tract organogenesis control homeotic regulator Barx1.