Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1

• DOI: 10.1242/dev.072728
• Publication Date: 2012-02-23T05:55:26Z
• Authors: Leonie Alten; Karin Schuster-Go...; Anja Beckers; Stephanie Groos; Bärbel Ulmer; Jan Hegermann; Matthias Ochs; Achim Gossler
• Keywords: Ciliogenesis; Motile cilium; Nodal signaling

The mouse transcription factor Noto is expressed in the node and controls morphogenesis, formation of nodal cilia left-right asymmetry. acts upstream Foxj1, which regulates ciliogenesis other tissues. However, significance Foxj1 for unclear; non-amniote species required structures equivalent to node. Here, we analyzed nodes, flow embryos replaced Noto-coding sequence with that or were deficient Foxj1. We show from locus functional restores structurally normal motile absence Noto. not sufficient correct positioning on cell surface within plane epithelium, cannot restore morphology. also essential Rfx3 Thus, function vertebrate organs asymmetry conserved, morphogenesis posterior localization cells independently