Quantitative cell polarity imaging defines leader-to-follower transitions during collective migration and the key role of microtubule-dependent adherens junction formation

• DOI: 10.1242/dev.101675
• Publication Date: 2014-03-04T12:26:41Z
• Authors: Céline Revenu; Sebastian Streichan; Erika Donà; Virginie Lecaudey; Lars Hufnagel; Darren Gilmour
• Keywords: Adherens junction; Cell polarity; Live cell imaging; Apical constriction; Morphogen; Polarity (international relations)

The directed migration of cell collectives drives the formation complex organ systems. A characteristic feature many migrating is a ‘tissue-scale’ polarity, whereby ‘leader’ cells at edge tissue guide trailing ‘followers’ that become assembled into polarised epithelial tissues en route. Here, we combine quantitative imaging and perturbation approaches to investigate state transitions during collective organogenesis, using zebrafish lateral line primordium as an in vivo model. readout three-dimensional based on centrosomal-nucleus axes, allows transition from leaders followers be quantitatively resolved for first time vivo. Using live reporters novel fluorescent protein timer approach, changes cell-cell adhesion underlying this by monitoring cadherin receptor localisation stability. This reveals while 2 expressed across entire tissue, functional apical junctions are zone progressively more stable leader-follower axis tissue. Perturbation experiments demonstrate these adherens requires dynamic microtubules. However, once stabilised, junction maintenance microtubule independent. Combined, data identify mechanism regulating leader-to-follower within collectives, relocation stabilisation cadherins, reveal key role microtubules process.