Bone formation following intrarenal transplantation of isolated murine chondrocytes: chondrocyte-bone cell transdifferentiation?

• DOI: 10.1242/dev.107.3.473
• Publication Date: 2021-04-26T00:42:55Z
• Authors: Stanislaw Moskale...; Jacek Malejczyk
• Keywords: Endochondral ossification; Chondrogenesis

ABSTRACT Isolated syngeneic epiphyseal chondrocytes transplanted into a muscle formed cartilage in which matrix resorption and endochondral ossification began at the end of second week after transplantation. After 56 days was converted an ossicle. In 7-day-old intrarenal transplants, nodules cartilage. 10-day-old islands bone appeared. Slight first noted 14-day-old transplants chondrocytes. eight weeks, contained mainly bone. Intramuscularly rib did not ossify. Nevertheless, appeared Bone allogeneic or chondrocytes, but such animals immunosuppressed by anti-thymocyte serum procarbazine. When spleen cells from immunized with were transferred to chondrocyte recipients two weeks transplantation, majority osteocytes dead. On other hand, intramuscular allogenic damaged sensitized cells. This suggested that 10- arose injected induction. To see whether some contaminating suspension, superficial layer removed collagenase digestion only more central used for Intrarenal these also yielded Furthermore, experiments stained toto Alcian blue Alizarin red S 7 14 transplantation examined under dissecting microscope. could be found while older ones present. These results strongly suggest transdifferentiated It seems which, their transdifferentiation, substituted matrix.