LIF, the ES-cell inhibition factor, reversibly blocks nephrogenesis in cultured mouse kidney rudiments

• DOI: 10.1242/dev.113.1.193
• Publication Date: 2021-04-23T18:25:23Z
• Authors: Jonathan B. L. Bard; Allyson S. A. Ross
• Keywords: Mesenchyme; Ureteric bud; Ingression; Organ culture; Embryonic Induction

Mouse kidney induction proceeds in vitro much as it does vivo: the ureteric bud bifurcates to give collecting ducts while mesenchyme condenses into aggregates which epithelialise and then elongate tubules with glomerular other nephron structures. We report here that factor known LIF (leukaemia inhibitory factor), regulates differentiation growth of embryonic-stem (ES) cells culture, has little effect on or ureteric-bud morphogenesis than stimulate bifurcation process. It however exert a striking mesenchyme. At about four times concentration required inhibit ES-cell differentiation, strongly but reversibly blocks effects metanephric induction: although around growing duct tips, number mature nephrons form over 6 days is reduced by 75% more. The few do develop presence probably come from already induced at time culture are indistinguishable those controls assayed morphology, X-gal staining endogenous galactosidase antibodies brush-border CD15 antigens. There further unexpected feature rudiments cultured absent controls: they contain an unexpectedly high stable epithelialised express laminin their periphery not further. These results argue process nephrogenesis involves least two distinct stages can be blocked LIF: initial future development aggregates.(ABSTRACT TRUNCATED AT 250 WORDS)