A quantitative and interspecific test for biological activity of anti-Müllerian hormone: the fetal ovary aromatase assay

• DOI: 10.1242/dev.114.3.721
• Publication Date: 2021-04-23T18:26:59Z
• Authors: Nathalie Di Clemente; Sarah Ghaffari; R. B. Pepinsky; C. Pieau; Nathalie Josso; R. L. Cate; B. Vigier
• Keywords: Anti-Müllerian hormone; Aromatase inhibitor

ABSTRACT Anti-Müllerian hormone (AMH), also known as Müllerian-inhibiting substance or factor, has previously been shown to sex-reverse the steroidogenic pattern of fetal mammalian ovaries through repression aromatase biosynthesis. Study ontogeny response cyclic AMP-stimulated activity rat AMH allowed us develop a quantitative biossay for hormone. Linear responses function logarithm concentration were observed over ranges 0.2 -7.5 μg/ml bovine protein and 0.15 -2 human protein, with maximal decrease in 90% both proteins. Under same vitro conditions, treatment did not affect testicular activity. Partially purified chick decreased ovarian activity, allowing use this test study gonads. Analysis species specificity indicated that turtle responded other vertebrate classes, whereas embryo could be repressed only by homospecific