Spatiotemporal oscillations of Notch1, Dll1 and NICD are coordinated across the mouse PSM

• DOI: 10.1242/dev.115535
• Publication Date: 2014-12-02T12:24:23Z
• Authors: Robert A. Bone; Charlotte S. L. B...; Guy Wiedermann; Zoltan Ferjentsik; Paul L. Appleton; Philip J. Murray; Miguel Maroto; J. Kim Dale
• Keywords: Somitogenesis; Paraxial mesoderm

During somitogenesis, epithelial somites form from the pre-somitic mesoderm (PSM) in a periodic manner. This periodicity is regulated by molecular oscillator, known as 'segmentation clock', that characterised an oscillatory pattern of gene expression sweeps PSM caudal-rostral direction. Key components segmentation clock are intracellular Notch, Wnt and FGF pathways, it widely accepted negative-feedback loops regulate expression. However, open question field how oscillations coordinated, spatiotemporal waves expression, across PSM. In this study, we provide potential mechanism for process. We show at mRNA level Notch1 receptor Delta-like 1 (Dll1) ligand vary dynamically both chick mouse. Remarkably, also demonstrate similar dynamics protein level; hence, pathway mediate intercellular coupling themselves exhibit dynamics. Moreover, quantify dynamic patterns Dll1 Notch1, they highly correlated with two [Lfng activated Notch (cleaved domain, NICD)]. Lastly, target signalling, whereas regulated. Regulation thus links activity main signalling pathways driving clock.