Sperm-inherited organelle clearance inC. elegansrelies on LC3-dependent autophagosome targeting to the pericentrosomal area

• DOI: 10.1242/dev.117879
• Publication Date: 2015-04-28T11:20:28Z
• Authors: Abderazak Djeddi; Sara Al Rawi; Jane Lynda Deuve; Charlene Perrois; Yu-Yu Liu; Marion Russeau; Martin Sachse; Vincent Galy
• Keywords: Autophagosome; Organelle; ATG8; Cell Cortex; Rab

Macroautophagic degradation of sperm-inherited organelles prevents paternal mitochondrial DNA transmission in C. elegans. The recruitment autophagy markers around sperm mitochondria has also been observed mouse and fly embryos but their role is debated. Both worm Atg8 ubiquitin-like proteins, LGG-1/GABARAP LGG-2/LC3, are recruited after fertilization. Whereas LGG-1 depletion affects autophagosome function, stabilizes the substrates lethal, we demonstrate that LGG-2 dispensable for formation participates microtubule-dependent transport toward pericentrosomal area prior to acidification. In absence LGG-2, autophagosomes remain clustered at cell cortex, away from centrosomes associated lysosomes. Thus, clearance delayed segregation between blastomeres prevented. This allowed us reveal a RAB-5/RAB-7 GTPases formation. conclusion, major contribution organelle resides its capacity mediate retrograde rather than fusion with acidic compartments: potential key function LC3 controlling fate other species.