Analyses of PS integrin functions during Drosophila development

• DOI: 10.1242/dev.118.3.737
• Publication Date: 2021-04-26T01:21:02Z
• Authors: Susan Zusman; Yevgenya Grinblat; Gene Yee; Fotis C. Kafatos; Richard O. Hynes
• Keywords: 0301 basic medicine; Integrins; 0303 health sciences; RNA Splicing; Pupa; Temperature; Genes, Insect; Exons; Eye; Muscle Development; Basement Membrane; Retina; 03 medical and health sciences; Drosophila melanogaster; Phenotype; Larva; Morphogenesis; Animals; Drosophila Proteins; Wings, Animal; Genes, Lethal; Integrin alpha Chains; Crosses, Genetic

ABSTRACT The Drosophila position-specific (PS) antigens are homologues of the vertebrate integrins, a family trans-membrane proteins that function in cell-matrix and cell-cell adhesion. common β subunit PS integrins (PSβ) is encoded by lethal(l)myospheroid gene (mys) required during wing, eye muscle development. By expressing PSβ protein at defined developmental periods, we have shown throughout pupation, but not earlier, for normal development wings. In contrast, key requirement occurs only late pupa. Furthermore, apparently differentiation ommatidial cells; their organization. These results consistent with roles interactions between wing epithelia two phases pupal expansion maintaining attachment fully formed fenestrated membrane to basement retina. We also examined functional significance alternative splicing transcript mys using P element-mediated transformation introduce transgenes producing one spliced forms PSβ. find either form sufficient rescue postem-bryonic phenotypes both splice necessary embryonic defects. This result indicates embryogenesis. location exons suggests integrin may interact α subunits and/or ligands.