Yap control of tissue growth relies on cell density and F-actin in zebrafish fin regeneration

• DOI: 10.1242/dev.119701
• Publication Date: 2015-07-25T02:44:43Z
• Authors: Rita Mateus; Raquel Lourenço; Yi Fang; Gonçalo Brito; Ana Farinho; Fábio Valério; Antonio Jacinto
• Keywords: Blastema; Hippo signaling pathway

Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema controlled precisely time and space. This allows gradual robust restoration of original size. However, how this established regulated not well understood. Here we report that Yap, Hippo pathway effector, chief player process: functionally manipulating Yap during dramatically affects cell expression key signaling pathways, impacting regenerative growth. The intracellular location tightly associated with different densities along proximal-distal axis, which correlate alterations morphology, cytoskeleton cell-cell contacts gradient-like manner. Importantly, inactivation occurs high density areas, conditional to F-actin distribution polymerization. We propose essential for its function dependent on mechanical tension, conferred balancing act activity.