Antisense oligodeoxynucleotides targeting PDGF-B mRNA inhibit cell proliferation during embryonic rat lung development

• DOI: 10.1242/dev.120.8.2163
• Publication Date: 2021-04-23T18:41:39Z
• Authors: Patricia Souza; Larisa Sedlackova; Maciej Kuliszewski; Jinxia Wang; Jason Liu; Irene Tseu; Mingyao Liu; A. Keith Tanswell; Martin Post
• Keywords: Platelet-Derived Growth Factor; 0301 basic medicine; 0303 health sciences; Base Sequence; Immunoblotting; Molecular Sequence Data; Oligonucleotides, Antisense; Polymerase Chain Reaction; Rats; 03 medical and health sciences; Morphogenesis; Animals; Rats, Wistar; Lung; Cell Division; DNA Primers

ABSTRACT There is increasing evidence to suggest that plateletderived growth factor (PDGF) or PDGF-like molecules play a role in fetal lung morphogenesis. Our previous studies demonstrated the presence of PDGF-AA and PDGF-BB homodimers embryonic rat lung. To explore further for development, we conducted intervention using PDGF-B chain-specific antisense oligodeoxynucleotides simple explant system. Unmodified inhibited, concentration-dependent manner, DNA synthesis A maximal inhibition 50% was observed. The inhibitory effect on reversed by addition exogenous but not PDGF-AA. Antisense treatment decreased protein content, as assessed immunoblot analyses. Incubation explants with neutralizing antibodies also resulted an synthesis. Morphometric analyses antisense-treated cultures showed significant reduction size when compared control cultures. epithelial component lungs specifically reduced, both mass labelling index, treatment. number terminal buds significantly affected Scrambled had no effect. These data involved regulating growth, degree branching,