The GAGA factor is required in the early Drosophila embryo not only for transcriptional regulation but also for nuclear division

• DOI: 10.1242/dev.122.4.1113
• Publication Date: 2021-04-26T02:37:44Z
• Authors: Krishna Moorthi Bhat; Gabriella Farkas; Francois Karch; Henrik Gyurkovics; Janos Gausz; Paul Schedl
• Keywords: Ultrabithorax

ABSTRACT The GAGA protein of Drosophila was first identified as a stimulatory factor in vitro transcription assays using the engrailed and Ultrabithorax promoters. Subsequent studies have suggested that promotes by blocking repressive effects histones; moreover, it has been shown to function chromatin remodeling, acting together with other factors formation nuclease hypersensitive sites vitro. is encoded Trithorax-like locus reported here we used maternal effect allele Trl13C examine functions during embryogenesis. We find required for proper expression variety developmental loci contain binding their upstream regulatory regions. observed disruptions gene are consistent those expected involved remodeling. In addition facilitating expression, appears more global role chromosome structure function. This spectrum nuclear cleavage cycle defects embryos. These include asynchrony cycles, failure condensation, abnormal segregation fragmentation. likely be related association heterochromatic satellite sequences which throughout cell cycle.